Abstract: Objective To analyze the correlation of miR-3934-5p expression with the clinical features and prognosis in patients with gastric cancer(GC). Methods In this study, the data sets of miR-3934-5p were got from the Cancer Genome Atlas(TCGA) database. TargetScan, miRDB, miRWalk, and DIANA were used to predict target genes of miR-3934-5p. Cytoscape software was used to identify the hub genes, and gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis were performed. Bioinformatics methods were used to explore the relationship between the expression of miR-3934-5p and the clinical characteristics of the GC patients. Results The expression level ofmiR-3934-5p in GC tissues was significantly higher than that in normal tissues(P<0.001). The high expression of miR-3934-5p was related to age(>65 vs ≤65,OR=1.901, P<0.001) and T stage(T3&T4 vs T1&T2,OR=0.585, P=0.015). Receiver operation characteristic curve analysis showed that miR-3934-5p could distinguish GC patients obviously(area under the curve =0.808). Survival analysis showed that elderly GC patients with high expression of miR-3934-5p had worse prognosis(P=0.024). The GO/KEGG enrichment analysis showed that the target genes of miR-3934-5p were involved in cell signal transduction, cell cycle, cellular senescence, DNA replication, homologous recombination and other processes mediated by p53, which might be involved in the occurrence and development of GC in miR-3934-5p up-regulated patients. Conclusions The expression of miR-3934-5p is up-regulated in GC patients, and miR-3934-5p may be involved in the occurrence and progression of GC. It may be used as a biomarker for diagnosing and predicting prognosis of GC.

Key words: gastric cancer, miR-3934-5p, aged, bioinformatics