Klotho、白细胞介素-8在老年慢性鼻窦炎病人鼻窦黏膜中的表达及与鼻息肉的关系

doi:10.3969/j.issn.1003-9198.2022.06.004

Abstract

Abstract: Objective To investigate the expression and the relationship with nasal polyps of Klotho and interleukin-8 (IL-8) in nasal mucosa of the elderly patients with chronic rhinosinusitis (CRS). Methods From September 2017 to September 2020, 80 elderly patients with CRS who underwent endoscopic surgery in our hospital were selected and divided into CRS without nasal polyps (CRSsNP) group (n=37) and CRS with nasal polyps (CRSwNP) group (n=43). The clinical data were collected. The expression level of Klotho protein was detected by Western blot, and the expression levels of Klotho mRNA and IL-8 mRNA were detected by real-time fluorescent quantitative polymerase chain reaction. Pearson correlation coefficient was used to describe the relationship of the expression of Klotho mRNA and IL-8 mRNA with the proportions of neutrophils and eosinophils in the elderly patients with CRS; and the risk factors of nasal polyps in the elderly patients with CRS were analyzed by multivariate Logistic regression analysis. Results Compared with CRSsNP group, the proportion of peripheral blood neutrophils in CRSwNP group was significantly lower (P<0.01), and the proportion of eosinophils in CRSwNP group was significantly higher(P<0.01); The expression of Klotho protein and Klotho mRNA in CRSwNP group were significantly lower (P<0.001), and the expression of IL-8 mRNA in CRSwNP group was significantly higher (P<0.001). The expression of Klotho mRNA was negatively correlated with the expression of IL-8 mRNA and the proportion of eosinophils, but positively correlated with the proportion of neutrophils in the elderly patients with CRS. The expression of IL-8 mRNA was negatively correlated with the proportion of neutrophils, but positively correlated with the proportion of eosinophils in the elderly patients with CRS. The expression of Klotho mRNA and IL-8 mRNA in nasal mucosa were the influencing factors for the occurence of nasal polyps in the elderly patients with CRS. Conclusions The expression of Klotho mRNA is low and the expression of IL-8 mRNA is high in the nasal mucosa of the elderly patients with CRSwNP, which may be used as important indicators to evaluate the occurrence of nasal polyps in the elderly patients with CRS.

Key words: chronic rhinosinusitis, nasal polyps, Klotho, interleukin-8, aged

CLC Number:

R 765.41

HAO Jian-hai, ZHANG Jun-yao, WANG Min. Expression and the relationship with nasal polyps of Klotho and interleukin-8 in nasal mucosa of elderly patients with chronic rhinosinusitis[J]. Practical Geriatrics, 2022, 36(6): 560-564.

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Expression and the relationship with nasal polyps of Klotho and interleukin-8 in nasal mucosa of elderly patients with chronic rhinosinusitis

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