针刺对去卵巢大鼠罗伊氏乳杆菌丰度及相关炎症因子表达的影响

doi:10.3969/j.issn.1003-9198.2025.09.005

摘要/Abstract

摘要： 目的观察电针干预下去卵巢模型大鼠肠道罗伊氏乳杆菌丰度的变化,从罗伊氏乳杆菌参与调节骨骼健康的角度,探讨针刺防治绝经后骨质疏松症的可能机制。方法选取30只3月龄雌性SD清洁级大鼠,随机分为5组,其中6只为空白组,6只为假手术组,18只行双侧卵巢摘除术后随机平均分为模型组、定植组和电针组。定植组喂养抗生素2周后,在无菌水中加入罗伊氏乳杆菌,每周喂食3次。电针组取穴:(1)关元、三阴交(双);(2)肾俞(双)、后三里(双),两组穴位交替进行,每次电针刺激20 min,每日1次,每周连续干预5 d,共12周。空白组、假手术组和模型组仅常规饲养,不作特殊处理。12周后处死大鼠,应用骨密度仪统一测定右侧胫骨、股骨骨密度,采用ELISA法检测血清中TNF-α和IL-6水平,采用Western blot法检测骨匀浆中核因子κB受体活化因子配体(RANKL)蛋白的表达水平,采用实时荧光定量PCR(qPCR)法检测大鼠粪便中肠道菌群结构变化。结果与模型组比较,定植组、电针组的胫骨、股骨骨密度显著升高(P<0.05),TNF-α、IL-6、RANKL蛋白表达水平显著降低(P<0.05),罗伊氏乳杆菌的丰度显著富集(P<0.05)。结论电针干预可以提高去卵巢模型大鼠的骨密度,下调血清TNF-α、IL-6表达水平及RANKL蛋白表达水平,调节肠道的罗伊氏乳杆菌丰度。

关键词: 绝经后骨质疏松症, 骨密度, 炎症因子, 罗伊氏乳杆菌

Abstract: Objective To observe the changes in the abundance of Lactobacillus reuteri in ovariectomy model rats under the intervention of electroacupuncture, and to explore the possible mechanism of acupuncture in preventing and treating postmenopausal osteoporosis from the perspective of the involvement of Lactobacillus reuteri in regulating bone health. Methods Thirty 3-month-old female SD clean rats were selected and randomly divided into 5 groups, which were blank group, sham-operation group, model group, fixation group, and electroacupuncture group, with 6 rats in each group. The rats in model group, fixation group, and electroacupuncture group received bilateral ovary removal. Fixation group was fed with antibiotics for two weeks, and then Lactobacillus reuteri was added in sterile water and fed for three times per week. Electroacupuncture group received electroacupuncture at the following acupoints: (1) Guanyuan(CV4) + Sanyinjiao(SP6); (2) Shenshu(BL23) + Housanli(ST36), and the acupoints of the two groups were alternated, and electroacupuncture stimulation was performed for 20 min each time, once a day, 5 d per week for a total of 12 weeks. Blank group, sham-operation group and model group only received routine rearing without any special treatments. After 12 weeks, the rats were put to death, and the bone mineral densities (BMDs) of right tibia and femur were determined uniformly by bone densitometer, and the serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were detected by enzyme linked immunosorbent assay (ELISA).The expression of receptor activator of nuclear factor-κB ligand (RANKL) protein in bone homogenate was detected by Western blot, and the structural changes of intestinal flora in the feces of rats were detected by real time quantative polymerase chain reaction. Results Compared with the model group, the BMDs of tibia and femur were significantly increased (P<0.05), the levels of TNF-α, IL-6 and RANKL protein were significantly decreased, and the abundance of Lactobacillus reuteri showed a significant enrichment (P<0.05) in the colonization group and the electroacupuncture group. Conclusions Electroacupuncture intervention can increase the BMD of ovariectomy model rats, down-regulate the expression levels of serum TNF-α, IL-6 and RANKL proteins, and regulate the abundance of Lactobacillus reuteri in the intestinal tract of ovariectomy model rats.

Key words: postmenopausal osteoporosis, bone mineral density, inflammatory factor, Lactobacillus reuteri

中图分类号:

R 589

张楚穹, 欧阳钢, 姜劲峰. 针刺对去卵巢大鼠罗伊氏乳杆菌丰度及相关炎症因子表达的影响[J]. 实用老年医学, 2025, 39(9): 883-887.

ZHANG Chuqiong, OUYANG Gang, JIANG Jinfeng. Effect of acupuncture on the abundance of Lactobacillus reuteri and expression of related inflammatory factors in ovariectomy model rats[J]. Practical Geriatrics, 2025, 39(9): 883-887.

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