Anillin蛋白在老年胰腺癌病人组织中的表达及其对胰腺癌细胞的作用机制研究

doi:10.3969/j.issn.1003-9198.2025.09.006

摘要/Abstract

摘要： 目的探讨Anillin蛋白(ANLN)在老年胰腺癌病人组织中的表达情况,以及ANLN通过调节蛋白激酶B(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路对胰腺癌细胞增殖、侵袭、迁移和凋亡的影响。方法收集86例老年胰腺癌病人组织标本,分析ANLN表达水平与临床病理特征的关系。采用实时荧光定量PCR(qPCR)检测胰腺癌组织、癌旁正常组织及细胞(HPDE6-C7、HS-766 T、PANC-1、SW1990和BxPC-3)中ANLN、Akt、mTOR mRNA的表达水平。选取 SW1990 细胞进行分组,分别为:空白组、阴性干扰组(NC siRNA 组)、ANLN 干扰组(ANLN siRNA 组)、Akt 活化组(SC79 组)、ANLN 干扰及 Akt 活化组(ANLN siRNA+SC79 组)。采用克隆形成实验、CCK-8 法、划痕实验和 Transwell 实验分别检测各组 SW1990 细胞的增殖、迁移及侵袭能力;采用流式细胞术检测各组SW1990细胞凋亡情况;采用qPCR检测各组SW1990细胞中ANLN、Akt、mTOR mRNA 的表达水平;采用Western blot检测各组SW1990细胞中的ANLN、Akt、p-Akt、mTOR、p-mTOR、磷脂酰肌醇3-激酶(PI3K)、p-PI3K蛋白的表达水平。结果胰腺癌组织中ANLN阳性表达与肿瘤病理分期、淋巴结转移及远处转移显著相关(P<0.05),而与性别、年龄、肿瘤原发部位无显著相关性(P＞0.05)。胰腺癌组织及细胞中ANLN、Akt、mTOR mRNA表达显著上调(P<0.05)。与空白组及NC siRNA组相比,ANLN siRNA组的细胞集落形成数量、细胞侵袭及迁移能力显著降低;ANLN、Akt、mTOR mRNA表达及ANLN、p-Akt/Akt、p-PI3K/PI3K、p-mTOR/mTOR蛋白的表达亦显著降低;细胞凋亡显著增加(P均<0.05)。与ANLN siRNA组相比,ANLN siRNA+SC79组的细胞集落形成数量、细胞侵袭及迁移能力显著增加;ANLN、Akt、mTOR mRNA表达及ANLN、p-Akt/Akt、p-PI3K/PI3K、p-mTOR/mTOR蛋白表达亦显著增加;细胞凋亡显著减少(P均<0.05)。结论 ANLN在老年胰腺癌病人组织中高表达。干扰ANLN可抑制胰腺癌细胞增殖、侵袭及迁移,可能与影响Akt/mTOR信号通路有关。

关键词: ANLN, 老年胰腺癌, 侵袭, 凋亡, Akt/mTOR信号通路

Abstract: Objective To study the expression of ANLN in tissues of elderly patients with pancreatic cancer and its mechanism of action on pancreatic cancer cells by regulating Akt/mTOR signaling pathway. Methods Tissue specimens were collected from 86 elderly patients with pancreatic cancer.The expression levels of ANLN, Akt and mTOR mRNA in the pancreatic cancer tissues, normal tissues and cells (HPDE6-C7, HS-766 T, PANC-1, SW1990 and BxPC-3) were detected by quantitative real-time polymerase chain reaction (qPCR). SW1990 cells were divided into blank group, NC siRNA group, ANLN siRNA group, SC79 group, and ANLN siRNA+SC79 group. The proliferation, cell migration and invasion of SW1990 cells were detected by clone formation experiment, CCK-8 method, Scratch experiments and Transwell experiments; Flow cytometry was applied to detect the cell apoptosis; qPCR was applied to detect the expression levels of ANLN, Akt, and mTOR mRNA; Western blot was applied to detect the expression levels of ANLN, Akt, PI3K, mTOR, p-Akt, p-PI3K and p-mTOR proteins. Results The expression level of ANLN was associated with the pathological stage, lymphatic metastasis and distant metastasis of tumors. The expression levels of ANLN, Akt and mTOR mRNA in pancreatic cancer tissues and cells increased obviously (P<0.05). Compared with blank group and NC siRNA group, the colony count, cell invasion and migration ability, the expression levels of ANLN mRNA, Akt mRNA, mTOR mRNA, ANLN protein, p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR were obviously decreased, and the cell apoptosis rate was obviously increased in ANLN siRNA group (P<0.05). Compared with ANLN siRNA group, the colony count, cell invasion and migration ability, the expression levels of ANLN mRNA, Akt mRNA, mTOR mRNA, ANLN protein, p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR were obviously increased, and cell apoptosis rate was obviously decreased in ANLN siRNA+SC79 group (P<0.05). Conclusions ANLN is highly expressed in the elderly patients with pancreatic cancer.Interference with ANLN can inhibit the proliferation, cell migration and invasion of pancreatic cancer cells, which may be related to the inhibition of Akt/mTOR signaling pathway.

Key words: ANLN, aged pancreatic cancer, invasion, apoptosis, Akt/mTOR signaling pathway

中图分类号:

R 735.9

钱静, 严晓娣, 沈超艳, 姚宁华, 石宇, 卞银珠. Anillin蛋白在老年胰腺癌病人组织中的表达及其对胰腺癌细胞的作用机制研究[J]. 实用老年医学, 2025, 39(9): 888-893.

QIAN Jing, YAN Xiaodi, SHEN Chaoyan, YAO Ninghua, SHI Yu, BIAN Yinzhu. Study on the expression of ANLN in tissues of elderly patients with pancreatic cancer and its mechanism of action on pancreatic cancer cells[J]. Practical Geriatrics, 2025, 39(9): 888-893.

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