Klotho、白细胞介素-8在老年慢性鼻窦炎病人鼻窦黏膜中的表达及与鼻息肉的关系

doi:10.3969/j.issn.1003-9198.2022.06.004

摘要/Abstract

摘要： 目的探讨老年慢性鼻窦炎(CRS)病人鼻窦黏膜组织中Klotho、IL-8的表达及与鼻息肉的关系。方法选取2017年9月至2020年9月在本院接受鼻内窥镜下手术治疗的80例老年CRS病人,分为CRS不伴鼻息肉(CRSsNP)组37例和CRS伴鼻息肉(CRSwNP)组43例。收集临床资料,采用免疫印迹法检测组织中Klotho蛋白表达水平,采用实时荧光定量PCR检测组织中Klotho mRNA、IL-8 mRNA的表达水平。采用Pearson相关系数描述老年CRS病人鼻窦黏膜组织Klotho mRNA、IL-8 mRNA表达与中性粒细胞比例、嗜酸性粒细胞比例的关系;采用多因素Logistic回归分析影响老年CRS病人发生鼻息肉的危险因素。结果 CRSwNP组外周血中性粒细胞比例明显低于CRSsNP组(P＜0.01),嗜酸性粒细胞比例明显高于CRSsNP组(P＜0.01)。CRSwNP组病人鼻窦黏膜组织Klotho蛋白、Klotho mRNA的表达水平均明显低于CRSsNP组(P＜0.001),IL-8 mRNA表达水平明显高于CRSsNP组(P＜0.001)。老年CRS病人鼻窦黏膜组织Klotho mRNA表达水平与IL-8 mRNA表达水平、嗜酸性粒细胞比例呈负相关,与中性粒细胞比例呈正相关;IL-8 mRNA表达水平与中性粒细胞比例呈负相关,与嗜酸性粒细胞比例呈正相关。鼻窦黏膜组织中Klotho mRNA、IL-8 mRNA的表达水平是老年CRS病人发生鼻息肉的影响因素。结论老年CRSwNP病人鼻窦黏膜组织中Klotho mRNA低表达、IL-8 mRNA高表达,两者有望成为评估老年CRS鼻息肉发生的重要指标。

关键词: 慢性鼻窦炎, 鼻息肉, Klotho, 白细胞介素-8, 老年人

Abstract: Objective To investigate the expression and the relationship with nasal polyps of Klotho and interleukin-8 (IL-8) in nasal mucosa of the elderly patients with chronic rhinosinusitis (CRS). Methods From September 2017 to September 2020, 80 elderly patients with CRS who underwent endoscopic surgery in our hospital were selected and divided into CRS without nasal polyps (CRSsNP) group (n=37) and CRS with nasal polyps (CRSwNP) group (n=43). The clinical data were collected. The expression level of Klotho protein was detected by Western blot, and the expression levels of Klotho mRNA and IL-8 mRNA were detected by real-time fluorescent quantitative polymerase chain reaction. Pearson correlation coefficient was used to describe the relationship of the expression of Klotho mRNA and IL-8 mRNA with the proportions of neutrophils and eosinophils in the elderly patients with CRS; and the risk factors of nasal polyps in the elderly patients with CRS were analyzed by multivariate Logistic regression analysis. Results Compared with CRSsNP group, the proportion of peripheral blood neutrophils in CRSwNP group was significantly lower (P<0.01), and the proportion of eosinophils in CRSwNP group was significantly higher(P<0.01); The expression of Klotho protein and Klotho mRNA in CRSwNP group were significantly lower (P<0.001), and the expression of IL-8 mRNA in CRSwNP group was significantly higher (P<0.001). The expression of Klotho mRNA was negatively correlated with the expression of IL-8 mRNA and the proportion of eosinophils, but positively correlated with the proportion of neutrophils in the elderly patients with CRS. The expression of IL-8 mRNA was negatively correlated with the proportion of neutrophils, but positively correlated with the proportion of eosinophils in the elderly patients with CRS. The expression of Klotho mRNA and IL-8 mRNA in nasal mucosa were the influencing factors for the occurence of nasal polyps in the elderly patients with CRS. Conclusions The expression of Klotho mRNA is low and the expression of IL-8 mRNA is high in the nasal mucosa of the elderly patients with CRSwNP, which may be used as important indicators to evaluate the occurrence of nasal polyps in the elderly patients with CRS.

Key words: chronic rhinosinusitis, nasal polyps, Klotho, interleukin-8, aged

中图分类号:

R 765.41

郝建海, 张俊瑶, 王敏. Klotho、白细胞介素-8在老年慢性鼻窦炎病人鼻窦黏膜中的表达及与鼻息肉的关系[J]. 实用老年医学, 2022, 36(6): 560-564.

HAO Jian-hai, ZHANG Jun-yao, WANG Min. Expression and the relationship with nasal polyps of Klotho and interleukin-8 in nasal mucosa of elderly patients with chronic rhinosinusitis[J]. Practical Geriatrics, 2022, 36(6): 560-564.

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