miR-3934-5p在老年胃癌病人中的表达及临床意义

doi:10.3969/j.issn.1003-9198.2022.02.013

摘要/Abstract

摘要： 目的分析miR-3934-5p在胃癌(GC)病人中的表达及其与临床特征和预后的关系。方法本研究利用癌症基因组图谱(TCGA)数据库miR-3934-5p相关数据,TargetScan、miRDB、miRWalk和DIANA等数据库预测miR-3934-5p靶基因,Cytoscape软件识别关键基因,对关键靶基因进行基因本体(GO)/京都基因与基因组百科全书(KEGG)富集分析,综合使用生物信息学方法分析miR-3934-5p表达与GC病人临床特征的关系。结果 GC组织中miR-3934-5p的表达水平远高于正常组织中的表达水平(P<0.001)。miR-3934-5p的高表达与年龄(＞65岁比≤65岁,OR=1.901,P＜0.001)和T分期(T3&T4比T1&T2,OR=0.585,P=0.015)相关。ROC曲线分析表明miR-3934-5p可以较好地区分GC病人(AUC=0.808)。生存分析显示,高表达miR-3934-5p的老年GC病人预后更差(P=0.024)。GO/KEGG富集分析显示,miR-3934-5p的靶基因与p53介导的细胞信号转导途径、细胞周期、细胞衰老、DNA复制、同源重组等过程有关。结论 GC病人miR-3934-5p表达上调,miR-3934-5p可能参与GC的发生和进展,可作为GC诊断和评估病情预后的生物标志物。

关键词: 胃癌, miR-3934-5p, 老年人, 生物信息学

Abstract: Objective To analyze the correlation of miR-3934-5p expression with the clinical features and prognosis in patients with gastric cancer(GC). Methods In this study, the data sets of miR-3934-5p were got from the Cancer Genome Atlas(TCGA) database. TargetScan, miRDB, miRWalk, and DIANA were used to predict target genes of miR-3934-5p. Cytoscape software was used to identify the hub genes, and gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis were performed. Bioinformatics methods were used to explore the relationship between the expression of miR-3934-5p and the clinical characteristics of the GC patients. Results The expression level ofmiR-3934-5p in GC tissues was significantly higher than that in normal tissues(P<0.001). The high expression of miR-3934-5p was related to age(>65 vs ≤65,OR=1.901, P<0.001) and T stage(T3&T4 vs T1&T2,OR=0.585, P=0.015). Receiver operation characteristic curve analysis showed that miR-3934-5p could distinguish GC patients obviously(area under the curve =0.808). Survival analysis showed that elderly GC patients with high expression of miR-3934-5p had worse prognosis(P=0.024). The GO/KEGG enrichment analysis showed that the target genes of miR-3934-5p were involved in cell signal transduction, cell cycle, cellular senescence, DNA replication, homologous recombination and other processes mediated by p53, which might be involved in the occurrence and development of GC in miR-3934-5p up-regulated patients. Conclusions The expression of miR-3934-5p is up-regulated in GC patients, and miR-3934-5p may be involved in the occurrence and progression of GC. It may be used as a biomarker for diagnosing and predicting prognosis of GC.

Key words: gastric cancer, miR-3934-5p, aged, bioinformatics

中图分类号:

R 735.2

孙洁, 查秀芳, 张楠楠, 桑楠, 赵君宁, 何伟. miR-3934-5p在老年胃癌病人中的表达及临床意义[J]. 实用老年医学, 2022, 36(2): 158-163.

SUN Jie, ZHA Xiu-fang, ZHANG Nan-nan, SANG Nan, ZHAO Jun-ning, HE Wei. The expression and clinical significance of miR-3934-5p in elderly patients with gastric cancer[J]. Practical Geriatrics, 2022, 36(2): 158-163.

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