黄芪多糖对老年晚期胃癌病人外周血中调节性T细胞的影响

doi:10.3969/j.issn.1003-9198.2021.06.007

实用老年医学 ›› 2021, Vol. 35 ›› Issue (6): 564-567.doi: 10.3969/j.issn.1003-9198.2021.06.007

黄芪多糖对老年晚期胃癌病人外周血中调节性T细胞的影响

吕彩霞, 苑晓烨, 邵伟华, 林华, 王素星, 赵景茹, 张明明

052260 河北省石家庄市,河北省人民医院老年病科(吕彩霞,苑晓烨,邵伟华,林华,王素星);神经内科(赵景茹);检验科(张明明)

收稿日期:2020-07-07 出版日期:2021-06-28 发布日期:2021-06-29
通讯作者: 张明明, Email: zhangmingming197612@126.com
基金资助:
河北省自然科学基金青年科学基金资助项目(H2016307015);河北省医学科学研究重点课题计划资助项目(20180003)

Effects of astragalus polysaccharide on expression and function of regulatory T cells in elderly patients with advanced gastric cancer

LYU Cai-xia, YUAN Xiao-ye, SHAO Wei-hua, LIN Hua, WANG Su-xing, ZHAO Jing-ru, ZHANG Ming-ming

LYU Cai-xia, YUAN Xiao-ye, SHAO Wei-hua, LIN Hua, WANG Su-xing. Department of Geriatrics; ZHAO Jing-ru. Department of Neurology; ZHANG Ming-ming. Clinical Laboratory, Hebei General Hospital, Shijiazhuang 052260, China

Received:2020-07-07 Online:2021-06-28 Published:2021-06-29

摘要/Abstract

摘要： 目的探讨黄芪多糖对老年晚期胃癌病人外周血中调节性T细胞(regulatory T cells, Tregs)表达及功能的影响。方法收集60例老年晚期胃癌病人,随机平均分为对照组(5%葡萄糖注射液)、低剂量组(黄芪多糖125 mg)及高剂量组(黄芪多糖250 mg),各组均于用药前后,应用流式细胞仪检测病人外周血中CD4⁺CD25⁺Tregs及CD4⁺CD25⁺FoxP3⁺Tregs的表达,应用ELISA法检测病人外周血中Treg相关抗炎细胞因子IL-10和转化生长因子-β1(TGF-β1)的含量,比较2组用药前后的差异。结果高剂量组外周血CD4⁺CD25⁺Tregs及CD4⁺CD25⁺FoxP3⁺Tregs的表达较用药前均明显降低(P<0.05),而低剂量组及对照组未见明显减少。同时高剂量组的IL-10及TGF-β1水平较用药前明显减少(P<0.05);而对照组的IL-10及TGF-β1水平较用药前未见明显减少,低剂量组TGF-β1水平较用药前降低(P<0.05)。结论黄芪多糖可能通过降低Tregs的表达及功能减轻老年晚期胃癌病人的免疫抑制状态。

关键词: 黄芪多糖, 胃癌, 调节性T细胞, 免疫抑制

Abstract: Objective To investigate the effect of astragalus polysaccharide on regulatory T cells (Tregs) in the peripheral blood of the elderly patients with advanced gastric cancer. Methods A total of 60 elderly patients with advanced gastric cancer were randomly divided into control group, low-dose astragalus polysaccharides group and high-dose astragalus polysaccharides group. Flow cytometry was used to detect the expression of CD4⁺CD25⁺Tregs and CD4⁺CD25⁺FoxP3⁺Tregs in the peripheral blood before or after the treatment. The levels of interleukin-10 (IL-10) and transforming growth factor-β1(TGF-β1) were detected by enzyme linked immunosorbent assay method. Results The expressions of CD4⁺CD25⁺Tregs and CD4⁺CD25⁺FoxP3⁺Tregs in the peripheral blood of the high-dose group were significantly lower than those before the administration (P<0.05).However, the expressions of CD4⁺CD25⁺Tregs and CD4⁺CD25⁺FoxP3⁺Tregs were not significantly decreased in the low-dose group and the control group. At the same time, the levels of IL-10 and TGF-β1 in the high-dose group were significantly lower than those before the administration (P<0.05), while showing no significant changes in the control group. The level of TGF-β1 in the low-dose group was lower than that before administration(P<0.05). Conclusions Astragalus polysaccharide may reduce the immunosuppressive status of the elderly patients with advanced gastric cancer by reducing the expression and function of Tregs.

Key words: astragalus polysaccharide, gastric cancer, regulatory T cells, immunosuppression

中图分类号:

R 735.2

吕彩霞, 苑晓烨, 邵伟华, 林华, 王素星, 赵景茹, 张明明. 黄芪多糖对老年晚期胃癌病人外周血中调节性T细胞的影响[J]. 实用老年医学, 2021, 35(6): 564-567.

LYU Cai-xia, YUAN Xiao-ye, SHAO Wei-hua, LIN Hua, WANG Su-xing, ZHAO Jing-ru, ZHANG Ming-ming. Effects of astragalus polysaccharide on expression and function of regulatory T cells in elderly patients with advanced gastric cancer[J]. Practical Geriatrics, 2021, 35(6): 564-567.

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黄芪多糖对老年晚期胃癌病人外周血中调节性T细胞的影响

Effects of astragalus polysaccharide on expression and function of regulatory T cells in elderly patients with advanced gastric cancer

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