血液lncRNA 00707检测在胃癌辅助诊断中的临床价值

doi:10.3969/j.issn.1003-9198.2022.12.006

摘要/Abstract

摘要： 目的探讨外周血液中长链非编码RNA(lncRNA 00707)和癌胚抗原(CEA)、糖类抗原19-9(CA19-9)检测在胃癌病人中的临床价值。方法收集经病理确诊为胃癌的病人血清40例,以及同期健康体检者血清标本40例。采用基于实时荧光定量PCR(RT-qPCR)的方法检测血清lncRNA 00707的相对表达量,采用受试者工作特征曲线(ROC)评估血清lncRNA 00707、CEA及CA19-9等指标单独及联合检测对胃癌的诊断效能。结果 RT-qPCR检测lncRNA 00707表达的方法具有较高的重复性和稳定性。胃癌初诊病人血清lncRNA 00707明显高于健康体检者,且lncRNA 00707与CEA联合检测可以提高胃癌的诊断效能。胃癌病人术后血清lncRNA 00707表达水平下降。结论初诊胃癌病人血清lncRNA 00707的表达水平显著上调,检测血清lncRNA 00707水平可辅助诊断胃癌,有望成为胃癌诊断与治疗的新靶点。

关键词: 胃癌, 外周血血清, lncRNA 00707, 实时荧光定量PCR

Abstract: Objective To investigate the clinical value of long non-coding RNA (lncRNA 00707), carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) in peripheral blood in the diagnosis of gastric cancer in the elderly patients. Methods The serum samples of 40 patients with gastric cancer confirmed by pathology and 40 healthy subjects were collected. The relative expression level of serum lncRNA 00707 was detected by real-time fluorescence quantitative PCR (RT-qPCR). The levels of CEA, CA19-9 and the biochemical parameters were detected. The diagnostic efficacy of serum lncRNA 00707, CEA and CA19-9 alone and in combination was evaluated by receiver operating characteristic (ROC) curve. Results The expression of lncRNA 00707 was detected by RT-qPCR with high repeatability and stability. The serum level of LncRNA 00707 in the patients with newly diagnosed gastric cancer was significantly higher than that in the healthy subjects, and the combined detection of lncRNA 00707 and CEA could improve the diagnostic efficiency of gastric cancer. The expression level of lncRNA 00707 decreased in the patients with gastric cancer after surgery. Conclusions The expression level of serum lncRNA 00707 in the patients with newly diagnosed gastric cancer is significantly up-regulated. Detection of serum lncRNA 00707 can assist in the diagnosis of gastric cancer, and is expected to become a new target for the diagnosis and treatment of gastric cancer.

Key words: gastric cancer, peripheral blood, lncRNA 00707, real-time fluorescence quantitative PCR

中图分类号:

R735.2

史恒川, 孔姗. 血液lncRNA 00707检测在胃癌辅助诊断中的临床价值[J]. 实用老年医学, 2022, 36(12): 1207-1210.

SHI Heng-chuan, KONG Shan. Clinical value of serum lncRNA 00707 in the diagnosis of gastric cancer[J]. Practical Geriatrics, 2022, 36(12): 1207-1210.

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