实用老年医学

实用老年医学 ›› 2010, Vol. 24 ›› Issue (2): 128-131.

• 论著 • 上一篇    下一篇

辛伐他汀对动脉粥样硬化大鼠高迁移率族蛋白1表达的影响

  

  1. 中国人民解放军总医院第一附属医院干一科
  • 出版日期:2010-04-20 发布日期:2010-03-10

Effects of simvastatin on the expression of HMGB1 in atherosclerotic rats

  • Online:2010-04-20 Published:2010-03-10

被引次数

7

摘要：

目的观察辛伐他汀对动脉粥样硬化大鼠高迁移率族蛋白1（HMGB1）的蛋白及基因表达及斑块形态学的影响，以揭示辛伐他汀抑制炎症反应，对动脉粥样硬化的保护作用机制。方法30只大鼠随机分为3组：空白对照组、动脉粥样硬化组和辛伐他汀组，辛伐他汀灌胃进行干预,给药4周。免疫组织化学染色法观察大鼠主动脉血管动脉粥样硬化斑块内HMGB1的蛋白表达情况，实时聚合酶链反应法检测大鼠主动脉组织HMGB1的基因表达变化，酶联免疫吸附试验检测大鼠血清HMGB1蛋白表达。结果（1）HMGB1在动脉粥样硬化斑块内的表达呈强阳性，辛伐他汀组HMGB1表达较单纯动脉粥样硬化组减少。（2）与空白对照组比较，在动脉粥样硬化斑块内动脉粥样硬化组HMGB1基因表达水平明显升高，差异有显著性（P＜001）；辛伐他汀干预组HMGB1基因表达低于动脉粥样硬化组（P＜001），高于空白对照组（P＜001）。（3）与空白对照组比较，动脉粥样硬化组及辛伐他汀组血清HMGB1蛋白表达水平均明显升高，差异有显著性（P＜001）,辛伐他汀组血清HMGB1表达低于动脉粥样硬化组（P＜005）。结论（1）动脉粥样硬化大鼠主动脉血管HMGB1的蛋白及基因表达均明显增强。（2）辛伐他汀能够减轻动脉粥样硬化大鼠的斑块形成，降低HMGB1的蛋白及基因表达，通过减轻炎症反应的途径起到保护作用。

关键词: 高迁移率族蛋白1, 动脉粥样硬化, 辛伐他汀, 维生素D3

Abstract:

ＯｂｊｅｃｔｉｖｅTo observe the effects of simvastatin on the protein and gene expression of HMGB1 and morphology of the atherosclerotic plaques in atherosclerotic rats, and to discover the protective mechanism of simvastatin for atherosclerosis.ＭｅｔｈｏｄｓThirty rats were randomly divided into 3 groups:control group, atherosclerosis group and simvastatin group.The atherosclerotic rats in simvastatin group were treated with simvastatin for 4 weeks. The histopathology change and the protein and gene expression of HMGB1 in the atherosclerotic plaques of aorta were observed, and compared in three groups.Ｒｅｓｕｌｔｓ(1) Simvastatin could inhibit the formation of the atherosclerotic plaques obviously, and the area of plaques decreased evidently. The expression of HMGB1 was enhanced in the area infiltrated with inflammatory cells and in the matrix around the necrotic cells.(2)The HMGB1 mRNA expression in all atherosclerotic rats was significantly increased(P＜001 ); In the simvastatin group, the gene expression of HMGB1 was lower than that in atherosclerosis group, while higher than that in the control group.(3)The plasma level of HMGB1 was significantly higher in the atherosclerotic group than that in the simvastatin group and the control group(P＜005 or P＜001) .Ｃｏｎｃｌｕｓｉｏｎｓ(1) The HMGB1 expression is increased in the atherosclerosis group.(2)Simvastatin could alleviate the formation of the plaques in the atherosclerotic rats, decreasing the protein and gene expression of HMGB1, then protecting the vessels by alleviating the inflammation.

Key words: high mobility group box1 protein, atherosclerosis, simvastatin, Vitamin D3

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