老年结直肠癌肝转移病人IL-18、PRL-3表达与PI3K/AKT信号通路关系探究

doi:10.3969/j.issn.1003-9198.2023.07.009

摘要/Abstract

摘要： 目的探讨老年结直肠癌(CRC)肝转移病人IL-18、促肝再生磷酸酶蛋白(PRL-3)的表达水平与磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(AKT)信号通路关系。方法选取我院2019年6月至2021年6月100例老年CRC病人,其中肝转移病人34例,非肝转移病人66例。采用蛋白免疫印迹法检测病人癌组织及癌旁组织中IL-18、PRL-3、PI3K、AKT蛋白表达情况并进行比较分析。采用Logistic回归分析影响老年CRC肝转移的危险因素,Pearson相关系数评估肝转移病人IL-18、PRL-3表达与PI3K/AKT信号通路的关系。结果癌组织中IL-18、PRL-3、PI3K及AKT蛋白表达水平均高于癌旁组织(P＜0.05);肝转移病人癌组织中IL-18、PRL-3、PI3K及AKT蛋白表达水平均高于非肝转移病人(P＜0.05);肝转移病人癌灶浸润深度T3～T4、低中分化、淋巴结转移比例均高于非肝转移病人(P＜0.05);Logistic回归分析显示,IL-18、PRL-3、PI3K及AKT蛋白阳性表达、癌灶浸润深度T3～T4、低中分化、淋巴结转移均为老年CRC肝转移的独立危险因素(P＜0.05);Pearson相关分析显示,老年CRC肝转移病人癌组织中IL-18、PRL-3蛋白表达水平与PI3K、AKT蛋白表达水平呈正相关(P＜0.05)。结论老年CRC肝转移病人IL-18、PRL-3呈高表达状态,两者表达水平均与PI3K、AKT蛋白水平呈正相关,IL-18、PRL-3是老年CRC肝转移的独立危险因素。

关键词: 结直肠癌, 老年人, 肝转移, 白细胞介素-18, 促肝再生磷酸酶蛋白, 磷脂酰肌醇-3-激酶, 蛋白激酶B

Abstract: Objective To explore the relationship of interleukin-18 (IL-18) and phosphatase of regenerating liver-3 (PRL-3) with phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) signaling pathways in the elderly patients with colorectal cancer (CRC) complicated with liver metastases. Methods A total of 100 elderly patients with CRC in our hospital were enrolled from June 2019 to June 2021. The expressions of IL-18, PRL-3, PI3K and AKT proteins in cancer tissues and para-carcinoma tissues were detected by Western Blot and compared. According to presence or absence of liver metastases, all patients were divided into metastases group (n=34) and non-metastases group (n=66). The risk factors of liver metastases were analyzed by Logistic regression analysis. The relationship between IL-18, PRL-3 and PI3K/AKT signaling pathways in liver metastases patients was analyzed by Pearson correlation. Results The expression levels of IL-18, PRL-3, PI3K and AKT proteins in cancer tissues were higher than those in para-carcinoma tissues (P<0.05), which were also higher in metastases group than those in non-metastases group (P<0.05). The proportions of cases with tumor invasion depth of T3-T4, low and moderate differentiation and lymph node metastasis in metastases group were higher than those in non-metastases group (P<0.05). Logistic regression analysis showed that the positive expressions of IL-18, PRL-3, PI3K and AKT proteins, tumor invasion depth of T3-T4, low and moderate differentiation, and lymph node metastasis were all independent risk factors of liver metastases in the elderly patients with CRC (P<0.05). Pearson correlation analysis showed that the expressions of IL-18 and PRL-3 proteins were positively correlated with the expressions of PI3K and AKT proteins in liver metastases patients (P<0.05). Conclusions The expressions of IL-18 and PRL-3 are up-regulated in the elderly patients with CRC and liver metastases, which are positively correlated with PI3K and AKT proteins. IL-18 and PRL-3 are independent risk factors of liver metastases in the elderly patients with CRC.

Key words: colorectal cancer, aged, liver metastasis, interleukin-18, phosphatase of regenerating liver-3, phosphatidylinositol-3-kinase, protein kinase B

中图分类号:

R 735.3

王雪, 邱婷, 吕颐菲, 高淑娟, 郭雪艳. 老年结直肠癌肝转移病人IL-18、PRL-3表达与PI3K/AKT信号通路关系探究[J]. 实用老年医学, 2023, 37(7): 680-684.

WANG Xue, QIU Ting, LYU Yi-fei, GAO Shu-juan, GUO Xue-yan. Relationship between IL-18, PRL-3 and PI3K/AKT signaling pathways in elderly patients with colorectal cancer complicated with liver metastases[J]. Practical Geriatrics, 2023, 37(7): 680-684.

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