实用老年医学

实用老年医学 ›› 2010, Vol. 24 ›› Issue (2): 118-121.

• 论著 • 上一篇    下一篇

α-硫辛酸对布比卡因所致小鼠神经细胞损伤的保护作用

  

  1. 210097江苏省南京市,南京师范大学生命科学院(王晓辉);210029江苏省南京市,南京医科大学第一附属医院老年医学科(姚玉珍，刘莉)；麻醉科(丁正年)
  • 出版日期:2010-04-20 发布日期:2010-03-10
  • 基金资助:

    国家自然科学基金(30972856);江苏省自然科学基金(BK2007247、BK2008467);江苏省“六大人才”基金(07-B-041)

Attenuation of bupivacaineinduced injury by α-Lipoic acid in mouse neuroblastoma cells

  • Online:2010-04-20 Published:2010-03-10

摘要：

目的探讨布比卡因(bupivacaine, Bup)的神经毒性以及α硫辛酸(αlipoic acid, LA)的保护作用。方法采用小鼠神经母细胞瘤细胞株N2a细胞培养模型，进行下列实验：(1) 观察Bup对N2a的损伤作用。将N2a细胞与不同浓度(0、300、600、900、1500、2000 μmol/L) 的Bup作用不同时间(12、24、36 h)，然后以MTT法和核固缩率分别评价细胞存活和细胞凋亡水平。每组实验重复6次。 (2) LA对Bup损伤的保护作用。将细胞以不同浓度(0、100、300、500、1000、2000 μmol/L)的LA预处理12 h，然后均再加入Bup (900 μmol/L)继续作用24 h。以MTT法评价细胞存活水平。每组实验重复6次。结果(1) Bup可显著损伤N2a细胞，并具剂量和时间依赖性。Bup浓度越大、作用时间越长，其神经毒性作用越强。(2) LA对Bup的神经损伤具有显著保护作用，其保护作用与剂量有关，本实验的最大保护剂量为500 μmol/L。结论Bup对N2a细胞有明显损伤作用，有剂量和时间双重依赖性，而LA可显著保护Bup所致的神经损伤。

关键词: 布比卡因, 神经损伤, &alpha, -硫辛酸

Abstract:

ＯｂｊｅｃｔｉｖｅTo investigate the neurotoxicity of bupivacaine (Bup) and the protective effects of αLipoic acid (LA). ＭｅｔｈｏｄｓMouse neuroblastoma N2a cells were employed in the present study. N2a cells were treated with variant concentrations of Bup (0, 300, 600, 900, 1500, 2000 μmol/L) for different durations (12, 24, 36 h). MTT assay and nuclear condensation analysis were used to evaluate the cell viability and apoptosis, respectively. N2a cells were pretreated with LA (0, 100, 300, 500, 1000, 2000 μmol/L) for 12 h, and followed by treatment with Bup (900 μmol/L) for 24 h. Cell viability was measured by MTT assay. Ｒｅｓｕｌｔｓ(1) Bup significantly induced injury in N2a cells, in both dose and timedependent manners. (2) LA markedly attenuated Bupinduced neurotoxicity in N2a cells, with the maximal protective efficiency at the concentration of 500 μmol/L.ＣｏｎｃｌｕｓｉｏｎｓTreatment of Bup could result in substantial injury in N2a cells. Pretreatment with LA could attenuate neurotoxicity induced by Bup.

Key words: bupivacaine, neurotoxicity, α-lipoic acid

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