几丁糖-胶原-丹参素缓释复合膜抑制兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制

doi:10.3969/j.issn.1003-9198.2025.07.004

Abstract

Abstract: Objective To investigate the mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue following lumbar laminectomy in rabbits. Methods A lumbar laminectomy model was established in rabbits using a surgical procedure. Different materials including normal saline(Model group), sodium hyaluronate (SH, M+SH group), chitosan-collagen composite membrane (CC, M+CC group), and chitosan-collagen-salvianolic acid slow-release composite membrane (CCS, M+ CCS group)were applied to the surgical area of the rabbit lumbar laminectomy model. The animals were sacrificed 4 or 8 weeks after operation. The apoptosis of lumbar vertebrae bone tissue was detected using TUNEL staining, and the expression levels of interleukin(IL)-1β, IL-6 mRNA and tumor necrosis factor (TNF)-α,transforming growth factor(TGF)-β1 protein at epidural scar tissue were analyzed using real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR) and Western blot (WB), respectively. Results Compared to Sham group,the number of apoptotic cells, the expression levels of IL-6 and IL-1β mRNA and TNF-α protein were significantly increased, and the expression level of TGF-β1 protein was significantly decreased in Model group 4 and 8 weeks after operation. Compared with Model group, the number of apoptotic cells,the expression levels of IL-6 and IL-1β mRNA and TNF-α protein were significantly decreased, and the expression level of TGF-β1 protein was significantly increased in M+CCS group (P<0.05) 4 and 8 weeks after operation. Conclusions The chitosan-collagen-salvianolic acid slow-release composite membrane can inhibit the inflammation at epidural scar tissue after lumbar laminectomy in rabbits by inhibiting the expression of pro-inflammatory factors (IL-1β、TNF-α and IL-6) and upregulating TGF-1β protein activity, thereby significantly reducing scar tissue cell apoptosis.

Key words: lumbar laminectomy, chitosan-collagen-salvianolic acid slow-release composite membrane, sodium hyaluronate, inflammatory response

CLC Number:

R681

WAN Xuan, KUANG Jun, GONG Jianbin, LIANG Chaoyi, WU Fan, SHU Zhimin, LIANG Weidong, WAN Xiaoming, YU Zhaozhong. Mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue after lumbar laminectomy in rabbits[J]. Practical Geriatrics, 2025, 39(7): 663-668.

References

[1] KIZILAY Z, CETIN N K, TOPCU A, et al. Effect of etanercept on the formation of epidural fibrosis in an experimental model[J]. Turk Neurosurg, 2018, 28(1): 111-117.
[2] TURAL EMON S, SOMAY H, ORAKDOGEN M, et al. Effects of hemostatic polysaccharide agent on epidural fibrosis formation after lumbar laminectomy in rats[J]. Spine J, 2016, 16(3): 414-419.
[3] SAE-JUNG S, JIRARATTANAPHOCHAI K. Prevention of peridural fibrosis using a cyclooxygenase-2 inhibitor (nonsteroidal anti-inflammatory drug) soaked in absorbable gelatin sponge: an experimental comparative animal model[J]. Spine, 2013, 38(16): E985-E991.
[4] YAMAN M E, ERGÜL G, GÜVENÇ Y, et al. Evaluation of topical dexmedetomidine administration in postlaminectomy epidural fibrosis rat model[J]. Int J Surg, 2018, 53: 80-85.
[5] FAN Q, WU H, KONG Q. Superhydrophilic PLGA-graft-PVP/PC nanofiber membranes for the prevention of epidural adhesion[J]. Int J Nanomedicine, 2022, 17: 1423-1435.
[6] VEDIAPPAN R S, MASCARENHAS A, NGUYEN-HOANG A, et al. Prevention of peridural adhesions in spinal surgery: assessing safety and efficacy of Chitogel with Deferiprone in a sheep model[J]. J Clin Neurosci, 2020, 72: 378-385.
[7] WANG B, LI P, SHANGGUAN L, et al. A novel bacterial cellulose membrane immobilized with human umbilical cord mesenchymal stem cells-derived exosome prevents epidural fibrosis[J]. Int J Nanomedicine, 2018, 13: 5257-5273.
[8] HAMMER M, DOLEYS D M, CHUNG O Y. Transforaminal ventral epidural adhesiolysis[J]. Pain Physician, 2001, 4(3): 273-279.
[9] SANDOVAL M A, HERNANDEZ-VAQUERO D. Preventing peridural fibrosis with nonsteroidal anti-inflammatory drugs[J]. Eur Spine J, 2008, 17(3): 451-455.
[10] BORA H, AYKOL S V, AKYÜREK N, et al. Inhibition of epidural scar tissue formation after spinal surgery: external irradiation vs. spinal membrane application[J]. Int J Radiat Oncol Biol Phys, 2001, 51(2): 507-513.
[11] GUO H, CALLAWAY J B, TING J P. Inflammasomes: mechanism of action, role in disease, and therapeutics[J]. Nat Med, 2015, 21(7): 677-687.
[12] RATHINAM V A, FITZGERALD K A. Inflammasome complexes: emerging mechanisms and effector functions[J]. Cell, 2016, 165(4): 792-800.
[13] NEURATH M F. Cytokines in inflammatory bowel disease[J]. Nat Rev Immunol, 2014, 14(5): 329-342.
[14] 况君, 余兆仲, 余清云, 等. 几丁糖-胶原-丹参素缓释复合膜预防大鼠脊髓损伤后硬膜黏连的作用[J]. 中华实验外科杂志, 2020, 37(8): 1498-1501.
[15] KIZILAY Z, CETIN K. Effect of methyl palmitate on the formation of epidural fibrosis in an experimental epidural fibrosis model[J]. J Invest Surg, 2018, 31(6): 469-474.
[16] DEMIREL E, YILDIZ K, ÇADIRCI K, et al. Effect of platelet-rich fibrin on epidural fibrosis and comparison to ADCON© Gel and hyaluronic acid[J]. Acta Orthop Traumatol Turc, 2018, 52(6): 469-474.
[17] YIU H H, GRAHAM A L, STENGEL R F. Dynamics of a cytokine storm[J]. PLoS One, 2012, 7(10): e45027.
[18] JIN R, NING X, LIU X, et al. Porphyromonas gingivalis-induced periodontitis could contribute to cognitive impairment in Sprague-Dawley rats via the P38 MAPK signaling pathway[J]. Front Cell Neurosci, 2023, 17: 1141339.
[19] DING L, WU L, CAO Y, et al. Modulating tumor-associated macrophage polarization by anti-maRCOMAb exerts anti-osteosarcoma effects through regulating osteosarcoma cell proliferation, migration and apoptosis[J]. J Orthop Surg Res, 2024, 19(1): 453.
[20] TURNER M D, NEDJAI B, HURST T, et al. Cytokines and chemokines: at the crossroads of cell signalling and inflammatory disease[J]. Biochim Biophys Acta, 2014, 1843(11): 2563-2582.
[21] TISONCIK J R, KORTH M J, SIMMONS C P, et al. Into the eye of the cytokine storm[J]. Microbiol Mol Biol Rev, 2012, 76(1): 16-32.
[22] TAY M Z, POH C M, RÉNIA L, et al. The trinity of COVID-19: immunity, inflammation and intervention[J]. Nat Rev Immunol, 2020, 20(6): 363-374.
[23] SAE-JUNG S, BUSARAKHAM N. The effect of adding carboxymethylcellulose and alginate to hyaluronic acid on reducing epidural fibrosis in a lumbar laminectomized rat model[J]. Acta Orthop Traumatol Turc, 2022, 56(4): 256-261.
[24] 曾瑞明, 况君, 万宣, 等. 几丁糖-胶原-丹参素缓释复合膜对兔腰椎板切除术后腰椎及硬膜外瘢痕组织的治疗作用[J]. 医学研究杂志, 2025, 54(2): 58-63.

Mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue after lumbar laminectomy in rabbits

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