核不均一核糖核蛋白C对肺腺癌细胞增殖、侵袭、迁移和凋亡的影响

doi:10.3969/j.issn.1003-9198.2025.01.007

摘要/Abstract

摘要： 目的探讨核不均一核糖核蛋白C (heterogeneous nuclear ribonucleoprotein C,HNRNPC)在肺腺癌细胞系中的表达以及敲低HNRNPC对肺腺癌细胞增殖、侵袭、迁移、凋亡的影响及可能机制。方法分别采用实时荧光定量PCR法(quantitative real-time PCR,qPCR)和蛋白免疫印迹法(western blot,WB)检测正常肺上皮细胞株BEAS-2B和肺腺癌细胞株A549中HNRNPC的mRNA和蛋白表达水平差异。利用慢病毒工具构建HNRNPC低表达稳转株,采用CCK-8法、平板克隆、Transwell实验、细胞划痕法研究敲低HNRNPC对A549细胞增殖、克隆形成、侵袭和迁移能力的影响;流式细胞术及WB检测敲低HNRNPC对A549细胞周期、凋亡和周期蛋白CDK1表达的影响。结果肺腺癌细胞株A549 HNRNPC mRNA及蛋白表达水平高于正常肺上皮细胞株。使用慢病毒工具敲低HNRNPC基因后,明显抑制了A549细胞的增殖、克隆形成能力,细胞侵袭和迁移能力显著下降,细胞周期阻滞和凋亡增加,CDK1蛋白表达水平下降。结论 HNRNPC在A549细胞中高表达,敲低HNRNPC可抑制A549细胞增殖、侵袭并诱导其凋亡。HNRNPC在肺腺癌中发挥着促癌基因的功能,有可能成为肺腺癌诊疗新的潜在靶点。

关键词: 核不均一核糖核蛋白C, 肺腺癌, 增殖, 侵袭, 迁移, 凋亡

Abstract: Objective To investigate the expression of heterogeneous nuclear ribonucleoprotein C (HNRNPC) in lung adenocarcinoma cell and the effect of knocking-down HNRNPC on the proliferation, invasion, migration, and apoptosis of lung adenocarcinoma cells line, as well as its possible mechanism. Methods The expression levels of mRNA and protein of HNRNPC in the normal lung epithelial cell line BEAS-2B were compared with those in the lung adenocarcinoma cell line A549 detected by quantitative real-time PCR (qPCR) and western blot (WB), respectively. Lentiviral tools were used to construct the low-expression stable transformation of HNRNPC. The effects of HNRNPC knockdown on the proliferation, clone formation, invasion, and migration of A549 cells were studied using CCK-8 method, plate cloning, Transwell assay and cell scratch assay. Flow cytometry and WB were employed to detect the effects of HNRNPC knockdown on cell cycle, apoptosis, and the expression of cyclin CDK1 in A549 cells. Results The expression levels of mRNA and protein of HNRNPC in lung adenocarcinoma cell lines were higher than those in normal lung epithelial cell lines. Knockdown of HNRNPC gene using lentiviral tools significantly inhibited the proliferation and clone formation ability of A549 cells, decreased the invasion and migration ability, increased cell cycle arrest and apoptosis, and reduced the expression level of CDK1 protein. Conclusions HNRNPC is highly expressed in A549 cells and plays a role of an oncogene in lung adenocarcinoma. Knocking-down HNRNPC can inhibit the proliferation, invasion, and induce apoptosis of A549 cells. HNRNPC may serve as a new potential target for the diagnosis and treatment of lung adenocarcinoma.

Key words: heterogeneous nuclear ribonucleoprotein C, lung adenocarcinoma, proliferation, invasion, migration, apoptosis

中图分类号:

R 734.2

吴凯悦, 宋一祎, 林欣, 朱珍, 孔志斌. 核不均一核糖核蛋白C对肺腺癌细胞增殖、侵袭、迁移和凋亡的影响[J]. 实用老年医学, 2025, 39(1): 26-31.

WU Kaiyue, SONG Yiyi, LIN Xin, ZHU Zhen, KONG Zhibin. Effect of HNRNPC on proliferation, invasion, migration, and apoptosis of lung adenocarcinoma cells[J]. Practical Geriatrics, 2025, 39(1): 26-31.

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