增龄对人体外周血CD4+CD25+Foxp3+调节T细胞、CD4+T细胞及细胞因子表达的影响

doi:10.3969/j.issn.1003-9198.2021.04.009

实用老年医学 ›› 2021, Vol. 35 ›› Issue (4): 354-357.doi: 10.3969/j.issn.1003-9198.2021.04.009

增龄对人体外周血CD4⁺CD25⁺Foxp3⁺调节T细胞、CD4⁺T细胞及细胞因子表达的影响

杨翔, 罗杰, 万文辉, 李翰卿, 贡歌, 张瑗, 陈阳希, 刘瑜, 张兴虎

210002 江苏省南京市,中国人民解放军东部战区总医院(原南京军区南京总医院)干部病房一科(杨翔,万文辉,贡歌,张瑗,陈阳希,刘瑜,张兴虎);保健科(罗杰);血液病科(李翰卿)

收稿日期:2020-06-05 发布日期:2021-04-25
通讯作者: 张兴虎,Email:zhangxh197002@163.com
基金资助:
全军保健专项课题(17BJZ17);国家自然科学基金青年项目(81701890)

Effect of aging on the expression of CD4⁺CD25⁺Foxp3⁺ regulatory T cells, CD4⁺T cells and cytokines in peripheral blood

YANG Xiang, WAN Wen-hui, GONG Ge, ZHANG Yuan, CHEN Yang-xi, LIU Yu, ZHANG Xing-hu, LUO Jie, LI Han-qing

YANG Xiang, WAN Wen-hui, GONG Ge, ZHANG Yuan, CHEN Yang-xi, LIU Yu, ZHANG Xing-hu. Department of Geriatrics; LUO Jie. Department of Cadre Health; LI Han-qing. Department of Hematology, Genernal Hospital of Eastern Theater Command, PLA, Nanjing 210002, China

Received:2020-06-05 Published:2021-04-25

摘要/Abstract

摘要： 目的探讨增龄对人体外周血CD4⁺CD25⁺Foxp3⁺调节T细胞(CD4⁺CD25⁺Foxp3⁺Treg)、 CD4⁺T细胞及细胞因子表达的影响。方法选择青年人(20~45岁)、中老年人(50~75岁)及高龄老年人(≥80岁)各40例,分别检测3组外周血CD4⁺T、CD4⁺CD25⁺Foxp3⁺Treg的绝对计数,并计算后者占前者的百分比,同时检测并比较3组人群外周血IL-2、干扰素-γ(IFN-γ)、TNF-α、IL-10和IL-17水平。结果高龄老年组CD4⁺T细胞绝对计数较中老年组与青年组显著下降(P<0.05);高龄老年组和中老年组外周血CD4⁺CD25⁺Foxp3⁺Treg绝对计数均明显高于青年组(P<0.05);中老年组CD4⁺CD25⁺Foxp3⁺Treg占CD4⁺T细胞百分比明显高于青年组,高龄老年组明显高于中老年组,差异均有统计学意义(P<0.05)。高龄老年组IL-2、IFN-γ和IL-17水平明显低于青年组和中老年组(P<0.05),中老年组IL-2明显低于青年组(P<0.05),高龄老年组IL-10水平明显高于青年组和中老年组(P<0.05),3组 TNF-α水平差异无统计学意义(P＞0.05)。结论中老年以后人体外周血CD4⁺CD25⁺Foxp3⁺Treg绝对计数明显增高,随着增龄,其占CD4⁺T细胞百分比逐渐升高。高龄老年人外周血CD4⁺T细胞绝对计数、IL-2、IFN-γ和IL-17水平明显下降, IL-10水平明显增高,说明老年人免疫功能进一步下降,衰老的微环境发生了改变。

关键词: 增龄, CD4⁺CD25⁺Foxp3⁺ 调节T细胞, CD4⁺T淋巴细胞, 细胞因子

Abstract: Objective To investigate the effects of aging on the expression of CD4⁺CD25⁺Foxp3⁺regulatory T cells (Treg), CD4⁺T cells in human peripheral blood and the levels of cytokines. Methods The absolute counts of CD4⁺T cells and CD4⁺CD25⁺Foxp3⁺Treg, and the levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), IL-10 and IL-17 were measured respectively in peripheral blood of 40 young persons (20-45 years old), 40 middle-aged persons (50-75 years old) and 40 elderly persons (above 80 years old), and also the percentage of CD4⁺CD25⁺Foxp3⁺Treg in CD4⁺T cells in peripheral blood of the three groups was calculated. The differences among the three groups were compared. Results The absolute count of CD4⁺T cells in peripheral blood decreased significantly in the elderly group than those in the young group and the middle-aged group(P<0. 05). The absolute count of CD4⁺CD25⁺Foxp3⁺Treg was significantly higher in peripheral blood in the elderly group and the middle-aged group than that in the young group(P<0. 05). The percentage of CD4⁺CD25⁺Foxp3⁺Treg in CD4⁺T cells was the highest in the elderly group, followed by the middle-aged group and the young group (P<0. 05). The levels of IL-2, IFN-γ and IL-17 in the elderly group were significantly lower than those in the young group and the middle-aged group (P<0. 05), and the level of IL-2 in the middle-aged group was significantly lower than that in the young group (P<0. 05). The level of IL-10 in the elderly group was significantly higher than that in the young group and the middle-aged group (P<0. 05), and the level of TNF-α among the three groups showed no significant difference (P>0. 05). Conclusions The absolute count of CD4⁺CD25⁺Foxp3⁺Treg in peripheral blood increases significantly after middle-age. With the increase of age, the ratio of CD4⁺CD25⁺Foxp3⁺Treg/CD4⁺T cells increases significantly. In the elderly, the absolute count of CD4⁺T cells and the levels of IL-2, IFN-γ, IL-17 decrease significantly, while the level of IL-10 significantly increases, indicating that the immune function of the elderly further decreases and the microenvironment is changed.

Key words: aging, CD4⁺CD25⁺Foxp3 regulatory T cell, CD4⁺T cells, cytokines

中图分类号:

R 392.7

杨翔, 罗杰, 万文辉, 李翰卿, 贡歌, 张瑗, 陈阳希, 刘瑜, 张兴虎. 增龄对人体外周血CD4⁺CD25⁺Foxp3⁺调节T细胞、CD4⁺T细胞及细胞因子表达的影响[J]. 实用老年医学, 2021, 35(4): 354-357.

YANG Xiang, WAN Wen-hui, GONG Ge, ZHANG Yuan, CHEN Yang-xi, LIU Yu, ZHANG Xing-hu, LUO Jie, LI Han-qing. Effect of aging on the expression of CD4⁺CD25⁺Foxp3⁺ regulatory T cells, CD4⁺T cells and cytokines in peripheral blood[J]. Practical Geriatrics, 2021, 35(4): 354-357.

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增龄对人体外周血CD4⁺CD25⁺Foxp3⁺调节T细胞、CD4⁺T细胞及细胞因子表达的影响

Effect of aging on the expression of CD4⁺CD25⁺Foxp3⁺ regulatory T cells, CD4⁺T cells and cytokines in peripheral blood

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增龄对人体外周血CD4+CD25+Foxp3+调节T细胞、CD4+T细胞及细胞因子表达的影响

Effect of aging on the expression of CD4+CD25+Foxp3+ regulatory T cells, CD4+T cells and cytokines in peripheral blood

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增龄对人体外周血CD4⁺CD25⁺Foxp3⁺调节T细胞、CD4⁺T细胞及细胞因子表达的影响

Effect of aging on the expression of CD4⁺CD25⁺Foxp3⁺ regulatory T cells, CD4⁺T cells and cytokines in peripheral blood