Anillin蛋白在老年胰腺癌病人组织中的表达及其对胰腺癌细胞的作用机制研究

doi:10.3969/j.issn.1003-9198.2025.09.006

Abstract

Abstract: Objective To study the expression of ANLN in tissues of elderly patients with pancreatic cancer and its mechanism of action on pancreatic cancer cells by regulating Akt/mTOR signaling pathway. Methods Tissue specimens were collected from 86 elderly patients with pancreatic cancer.The expression levels of ANLN, Akt and mTOR mRNA in the pancreatic cancer tissues, normal tissues and cells (HPDE6-C7, HS-766 T, PANC-1, SW1990 and BxPC-3) were detected by quantitative real-time polymerase chain reaction (qPCR). SW1990 cells were divided into blank group, NC siRNA group, ANLN siRNA group, SC79 group, and ANLN siRNA+SC79 group. The proliferation, cell migration and invasion of SW1990 cells were detected by clone formation experiment, CCK-8 method, Scratch experiments and Transwell experiments; Flow cytometry was applied to detect the cell apoptosis; qPCR was applied to detect the expression levels of ANLN, Akt, and mTOR mRNA; Western blot was applied to detect the expression levels of ANLN, Akt, PI3K, mTOR, p-Akt, p-PI3K and p-mTOR proteins. Results The expression level of ANLN was associated with the pathological stage, lymphatic metastasis and distant metastasis of tumors. The expression levels of ANLN, Akt and mTOR mRNA in pancreatic cancer tissues and cells increased obviously (P<0.05). Compared with blank group and NC siRNA group, the colony count, cell invasion and migration ability, the expression levels of ANLN mRNA, Akt mRNA, mTOR mRNA, ANLN protein, p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR were obviously decreased, and the cell apoptosis rate was obviously increased in ANLN siRNA group (P<0.05). Compared with ANLN siRNA group, the colony count, cell invasion and migration ability, the expression levels of ANLN mRNA, Akt mRNA, mTOR mRNA, ANLN protein, p-PI3K/PI3K, p-Akt/Akt, p-mTOR/mTOR were obviously increased, and cell apoptosis rate was obviously decreased in ANLN siRNA+SC79 group (P<0.05). Conclusions ANLN is highly expressed in the elderly patients with pancreatic cancer.Interference with ANLN can inhibit the proliferation, cell migration and invasion of pancreatic cancer cells, which may be related to the inhibition of Akt/mTOR signaling pathway.

Key words: ANLN, aged pancreatic cancer, invasion, apoptosis, Akt/mTOR signaling pathway

CLC Number:

R 735.9

QIAN Jing, YAN Xiaodi, SHEN Chaoyan, YAO Ninghua, SHI Yu, BIAN Yinzhu. Study on the expression of ANLN in tissues of elderly patients with pancreatic cancer and its mechanism of action on pancreatic cancer cells[J]. Practical Geriatrics, 2025, 39(9): 888-893.

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Study on the expression of ANLN in tissues of elderly patients with pancreatic cancer and its mechanism of action on pancreatic cancer cells

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