针刺干预对绝经后骨质疏松症模型大鼠肠道菌群影响的研究

doi:10.3969/j.issn.1003-9198.2022.02.006

Abstract

Abstract: Objective To observe the changes of intestinal flora related to bone metabolism in the postmenopausal osteoporosis model rats receiving the intervention of electroacupuncture. Methods A total of 32 3-month-old female SD rats were randomly divided into blank group, sham operation group, model group and electroacupuncture group, with 8 rats in each group. The rats in the model group and the electroacupuncture group underwent bilateral ovariectomy. The electroacupuncture group was alternately given electroacupuncture intervention at "Guanyuan(CV4) + Sanyinjiao(SP6)"(double) and "Shenshu(BL23) + Housanli(ST36)"(double) for 20 min each time, once a day, for 5 consecutive days every week for 12 weeks. After 12 weeks, the bone mineral density(BMD) of the right tibia and femur was measured by bone densitometer. The feces were taken for 16S rDNA flora detection. Results Compared with blank group and sham operation group, the level of BMD in model group was significantly decreased(P<0.05). Compared with model group, the level of BMD in electroacupuncture group was significantly increased(P<0.05). At the phylum level, the abundance of Tenericutes, Actinobacteria and Deferribacteria in model group increased significantly compared with blank group and sham operation group(P<0.05), while the abundance of Firmicutes, Bacteroidetes, Proteobacteria and Melainabacteria decreased(P<0.05). Compared with the model group, the abundance of Firmicutes, Bacteroidetes, Proteobacteria and Melainabacteria in electroacupuncture group increased(P<0.05), and the abundance of Tenericutes, Actinobacteria and Deferribacteria in electroacupuncture group decreased(P<0.05). At the genus level, the abundance of Bacteroides, Lactobacillus and Enterococcus in model group decreased(P<0.05) compared with blank group and sham operation group. Compared with model group, the abundance of Bacteroides, Lactobacillus and Enterococcus in electroacupuncture group increased. Conclusions Electroacupuncture can improve the level of BMD of ovariectomized rats and regulate their intestinal microflora structure.

Key words: postmenopausal osteoporosis, bone mineral density, intestinal flora

CLC Number:

R 589

ZHANG Chu-qiong, SHI Na, OUYANG Gang. Effect of acupuncture intervention on intestinal microflora of postmenopausal osteoporosis model rats[J]. Practical Geriatrics, 2022, 36(2): 129-133.

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Effect of acupuncture intervention on intestinal microflora of postmenopausal osteoporosis model rats

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