血清lncRNA NEAT1、SIRT2水平与老年急性缺血性脑卒中病人病情及预后的关系

doi:10.3969/j.issn.1003-9198.2025.06.013

摘要/Abstract

摘要： 目的探讨血清长链非编码RNA核富集丰富的转录本1(lncRNA NEAT1)、沉默信息调节因子2(SIRT2)水平与老年急性缺血性脑卒中(AIS)病人病情及预后的关系。方法选取2021年1月至2023年9月西安国际医学中心医院收治的老年AIS病人163例(AIS组)和同期健康体检老年人82例(对照组),根据病情将老年AIS病人分为轻度AIS组(32例)、中度AIS组(45例)、中重度AIS组(30例)、重度AIS组(56例),根据6个月预后分为预后不良组(52例)和预后良好组(111例)。分别采用实时荧光定量PCR和酶联免疫吸附法检测血清lncRNA NEAT1、SIRT2水平,采用Spearman相关分析老年AIS病人血清lncRNA NEAT1、SIRT2水平与NIHSS评分的相关性,多因素logistic回归分析老年AIS病人预后的影响因素。结果与对照组比较,AIS组血清lncRNA NEAT1、SIRT2水平升高(P均＜0.01);随着AIS病情逐渐加重,病人血清lncRNA NEAT1、SIRT2水平逐渐升高(P均＜0.05);老年AIS病人血清lncRNA NEAT1、SIRT2水平与NIHSS评分均呈正相关(r＝0.775、0.743,P均＜0.001)。多因素logistic回归分析显示,老年AIS病人不良预后的独立危险因素为梗死体积增加、NIHSS评分增加、lncRNA NEAT1水平升高和SIRT2水平升高(P均＜0.05)。结论血清lncRNA NEAT1、SIRT2水平升高与老年AIS病人病情加重和不良预后密切相关。

关键词: 老年人, 急性缺血性脑卒中, 长链非编码RNA核富集丰富的转录本1, 沉默信息调节因子2, 预后

Abstract: Objective To investigate the relationship of serum long non-coding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) and silent information regulator factor 2 (SIRT2) with the condition and prognosis of the elderly patients with acute ischemic stroke (AIS). Methods A total of 163 elderly AIS patients (AIS group) admitted to Xi’an International Medical Center Hospital from January 2021 to September 2023 and 82 healthy elderly people (control group) undergoing physical examination during the same period were enrolled in this study. The elderly patients with AIS were divided into mild AIS group (32 cases), moderate AIS group (45 cases), moderate-severe AIS group (30 cases), and severe AIS group (56 cases), and were divided into poor prognosis group (52 cases) and good prognosis group (111 cases) according to the 6-month prognosis. Real-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the serum levels of lncRNA NEAT1 and SIRT2, respectively. Spearman’s correlation was used to analyze the correlation of serum levels of lncRNA NEAT1 and SIRT2 with National Institute of Health Stroke Scale (NIHSS) scores. Multivariate logistic regression analysis was used to determine the risk factors for prognosis of the elderly patients with AIS. Results Compared with the control group, the serum levels of lncRNA NEAT1 and SIRT2 were higher in the AIS group (P＜0.01). As the disease gradually worsened, the serum levels of lncRNA NEAT1 and SIRT 2 gradually increased in the elderly AIS patients (all P＜0.05); Spearman’s correlation showed that the serum levels of lncRNA NEAT1 and SIRT2 were positively correlated with NIHSS scores in the elderly AIS patients (r＝0.775, 0.743, P＜0.01).The independent risk factors for poor prognosis in the elderly AIS patients were increased infarct volume, increased NIHSS score, elevated levels of lncRNA NEAT1 and SIRT2 (all P＜0.05). Conclusions Elevated serum lncRNA NEAT1 and SIRT2 levels are closely associated with exacerbation and poor prognosis in the elderly AIS patients.

Key words: aged, acute ischemic stroke, long non-coding RNA nuclear paraspeckle assembly transcript 1, silent information regulator factor 2-related enzyme 2, prognosis

中图分类号:

R743.3

鲁爽, 汪杰, 孙苗, 刘永平, 张凯丽, 廖鹏斌. 血清lncRNA NEAT1、SIRT2水平与老年急性缺血性脑卒中病人病情及预后的关系[J]. 实用老年医学, 2025, 39(6): 595-600.

LU Shuang, WANG Jie, SUN Miao, LIU Yongping, ZHANG Kaili, LIAO Pengbin. Relationship of serum lncRNA NEAT1 and SIRT2 levels with the condition and prognosis of elderly patients with acute ischemic stroke[J]. Practical Geriatrics, 2025, 39(6): 595-600.

参考文献

[1] GBD 2019 Stroke Collaborators. Global, regional, and national burden of stroke and its risk factors, 1990-2019: a systematic analysis for the global burden of disease study 2019[J]. Lancet Neurol,2021,20(10):795-820.
[2] 中国老年学和老年医学学会, 董欢欢, 吕东蔚.老年缺血性脑卒中慢病管理指南[J].中西医结合研究,2022,14(6):382-392.
[3] 中华医学会神经病学分会,中华医学会神经病学分会脑血管病学组.中国急性缺血性卒中诊治指南2023[J].中华神经科杂志,2024,57(6):523-559.
[4] 曾名望,钟瑞蓬,蓝青海,等.综述非编码RNA在缺血性脑卒中的作用机制研究进展[J].中风与神经疾病杂志,2022,39(12):1133-1136.
[5] 郑海建,王翎,刘广岚,等.长链非编码RNA NEAT1在急性缺血性脑卒中的诊断价值[J].中国老年学杂志,2022,42(7):1560-1562.
[6] LU W, JI H, WU D. SIRT2 plays complex roles in neuroinflammation neuroimmunology-associated disorders[J]. Front Immunol, 2023, 14:1174180.
[7] ANGERFORS A, BRÄNNMARK C, LAGGING C, et al. Proteomic profiling identifies novel inflammation-related plasma proteins associated with ischemic stroke outcome[J]. J Neuroinflammation,2023,20(1):224.
[8] 侯东哲,张颖,巫嘉陵,等.中文版美国国立卫生院脑卒中量表的信度与效度研究[J].中华物理医学与康复杂志,2012,34(5):372-374.
[9] 中华医学会,中华医学会杂志社,中华医学会全科医学分会,等.缺血性卒中基层诊疗指南(2021年)[J].中华全科医师杂志,2021,20(9):927-946.
[10] 张磊,刘建民.改良Rankin量表[J].中华神经外科杂志,2012,28(5):512.
[11] 任行龙,聂贝贝,马丽霞,等.老年急性缺血性脑卒中患者神经功能缺损加重的危险因素[J].海南医学,2023,34(24):3544-3547.
[12] 王明泽,曹勇.老年卒中外科治疗新进展[J].临床外科杂志,2023,31(11):1004-1007.
[13] 姜逍瑶,赵旭东,鲍磊,等.老年营养风险指数对急性缺血性脑卒中患者预后的预测价值[J].中华老年心脑血管病杂志,2023,25(1):35-38.
[14] 秦少博,王慧,李莹.LncRNA NEAT1在动脉粥样硬化大鼠模型中调节血管平滑肌细胞发育的机制[J].临床和实验医学杂志,2022,21(11):1125-1130.
[15] ZHENG H, ZHANG G, LIU G, et al. Up-regulation of lncRNA NEAT1 in cerebral ischemic stroke promotes activation of astrocytes by modulation of miR-488-3p/RAC1[J]. Exp Brain Res,2023,241(2):395-406.
[16] CHEN T, HUANG X, ZHAO Y X, et al. NEAT1 inhibits the angiogenic activity of cerebral arterial endothelial cells by inducing the M1 polarization of microglia through the AMPK signaling pathway[J]. Cell Mol Biol Lett,2024,29(1):62.
[17] CHEN J M, LI X L, YANG Y E, et al. Competing endogenous RNA network analysis of the molecular mechanisms of ischemic stroke[J]. BMC Genomics,2023,24(1):67.
[18] ZHOU Z W, REN X, ZHOU W S, et al. LncRNA NEAT1 alleviates ischemic stroke via transcriptional inhibition of NLRP3 mediated by the miR-10b-5p/BCL6 axis[J]. Acta Neurobiol Exp :Wars,2022,82(1):12-21.
[19] LIAN X W, LUO B. Knockdown of NEAT1 induced microglial M2 polarization via miR-374a-5p/NFAT5 axis to inhibit inflammatory response caused by OGD/R[J]. Acta Neurobiol Exp: Wars,2021,81(4):362-374.
[20] FANG J, WANG Z, MIAO C Y. Angiogenesis after ischemic stroke[J]. Acta Pharmacol Sin,2023,44(7):1305-1321.
[21] KREY L, LÜHDER F, KUSCH K, et al. Knockout of silent information regulator 2 (SIRT2) preserves neurological function after experimental stroke in mice[J]. J Cereb Blood Flow Metab,2015,35(12):2080-2088.
[22] YAO L, PENG P, DING T, et al. m⁶A-induced lncRNA MEG3 promotes cerebral ischemia-reperfusion injury via modulating oxidative stress and mitochondrial dysfunction by hnRNPA1/Sirt2 axis[J]. Mol Neurobiol,2024,2(15):1.
[23] WU D, LU W, WEI Z, et al.Neuroprotective effect of SIRT2-specific inhibitor AK-7 against acute cerebral ischemia is P38 activation-dependent in mice[J]. Neuroscience, 2018, 374: 61-69.