几丁糖-胶原-丹参素缓释复合膜抑制兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制

doi:10.3969/j.issn.1003-9198.2025.07.004

实用老年医学 ›› 2025, Vol. 39 ›› Issue (7): 663-668.doi: 10.3969/j.issn.1003-9198.2025.07.004

几丁糖-胶原-丹参素缓释复合膜抑制兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制

万宣, 况君, 龚剑斌, 梁超轶, 吴凡, 舒志敏, 梁卫东, 万小明, 余兆仲

330006 江西省南昌市,江西中医药大学附属医院脊柱科(万宣,况君);创伤骨科(龚剑斌,梁超轶,舒志敏,梁卫东,万小明,余兆仲);儿童骨科(吴凡)

收稿日期:2024-10-31 出版日期:2025-07-20 发布日期:2025-07-22
通讯作者: 余兆仲,Email:yuzhaozhong52@163.com
基金资助:
国家自然科学基金地区基金资助项目(82260943);万小明全国名老中医药专家传承工作室建设项目(国中医药人教函〔2022〕75号);江西省自然科学基金资助项目(20192BAB205097)

Mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue after lumbar laminectomy in rabbits

WAN Xuan, KUANG Jun, GONG Jianbin, LIANG Chaoyi, WU Fan, SHU Zhimin, LIANG Weidong, WAN Xiaoming, YU Zhaozhong

Department of Spine(WAN Xuan, KUANG Jun);Orthopedic Trauma (GONG Jianbin, LIANG Chaoyi, SHU Zhimin, LIANG Weidong, WAN Xiaoming, YU Zhaozhong); Pediatric Orthopedics (WU Fan), Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China

Received:2024-10-31 Online:2025-07-20 Published:2025-07-22
Contact: YU Zhaozhong, Email:yuzhaozhong52@163.com

摘要/Abstract

摘要： 目的探究几丁糖-胶原-丹参素缓释复合膜对兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制。方法采用手术法构建兔腰椎板切除模型,在腰椎板切除术区加入不同材料:生理盐水(Model组)、透明质酸钠(M+SH组)、几丁糖-胶原复合膜(M+CC组)、几丁糖-胶原-丹参素缓释复合膜(M+CCS组),分别于术后4周和8周处死动物,取腰椎骨组织行TUNEL染色检测凋亡情况,取硬膜外瘢痕组织行实时荧光定量PCR(qRT-PCR)检测IL-1β和IL-6 mRNA的表达,采用Western Blot检测TNF-α、转化生长因子-β1(TGF-β1)蛋白的表达。结果 Model组中,凋亡细胞数在4周和8周时较假手术组(Sham组)显著增多,IL-6和IL-1β的mRNA和TNF-α蛋白表达显著升高,TGF-β1蛋白表达显著下降;与Model组相比,4周和8周时M+SH组、M+CC组和M+CCS组凋亡细胞数量减少,M+CCS组IL-6和IL-1β的mRNA和TNF-α蛋白表达受到抑制,TGF-β1蛋白表达增加,差异均有统计学意义(P＜0.05)。结论几丁糖-胶原-丹参素缓释复合膜通过抑制促炎因子TNF-α、IL-1β、IL-6表达并上调TGF-β1蛋白活性,显著减少瘢痕组织细胞凋亡,从而有效抑制兔腰椎板切除术后硬膜外瘢痕组织的炎症反应。

关键词: 腰椎板切除术, 几丁糖-胶原-丹参素缓释复合膜, 透明质酸钠, 炎症反应

Abstract: Objective To investigate the mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue following lumbar laminectomy in rabbits. Methods A lumbar laminectomy model was established in rabbits using a surgical procedure. Different materials including normal saline(Model group), sodium hyaluronate (SH, M+SH group), chitosan-collagen composite membrane (CC, M+CC group), and chitosan-collagen-salvianolic acid slow-release composite membrane (CCS, M+ CCS group)were applied to the surgical area of the rabbit lumbar laminectomy model. The animals were sacrificed 4 or 8 weeks after operation. The apoptosis of lumbar vertebrae bone tissue was detected using TUNEL staining, and the expression levels of interleukin(IL)-1β, IL-6 mRNA and tumor necrosis factor (TNF)-α,transforming growth factor(TGF)-β1 protein at epidural scar tissue were analyzed using real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR) and Western blot (WB), respectively. Results Compared to Sham group,the number of apoptotic cells, the expression levels of IL-6 and IL-1β mRNA and TNF-α protein were significantly increased, and the expression level of TGF-β1 protein was significantly decreased in Model group 4 and 8 weeks after operation. Compared with Model group, the number of apoptotic cells,the expression levels of IL-6 and IL-1β mRNA and TNF-α protein were significantly decreased, and the expression level of TGF-β1 protein was significantly increased in M+CCS group (P<0.05) 4 and 8 weeks after operation. Conclusions The chitosan-collagen-salvianolic acid slow-release composite membrane can inhibit the inflammation at epidural scar tissue after lumbar laminectomy in rabbits by inhibiting the expression of pro-inflammatory factors (IL-1β、TNF-α and IL-6) and upregulating TGF-1β protein activity, thereby significantly reducing scar tissue cell apoptosis.

Key words: lumbar laminectomy, chitosan-collagen-salvianolic acid slow-release composite membrane, sodium hyaluronate, inflammatory response

中图分类号:

R681

万宣, 况君, 龚剑斌, 梁超轶, 吴凡, 舒志敏, 梁卫东, 万小明, 余兆仲. 几丁糖-胶原-丹参素缓释复合膜抑制兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制[J]. 实用老年医学, 2025, 39(7): 663-668.

WAN Xuan, KUANG Jun, GONG Jianbin, LIANG Chaoyi, WU Fan, SHU Zhimin, LIANG Weidong, WAN Xiaoming, YU Zhaozhong. Mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue after lumbar laminectomy in rabbits[J]. Practical Geriatrics, 2025, 39(7): 663-668.

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几丁糖-胶原-丹参素缓释复合膜抑制兔腰椎板切除术后硬膜外瘢痕组织炎症的作用机制

Mechanism of chitosan-collagen-salvianolic acid slow-release composite membrane inhibiting inflammation in epidural scar tissue after lumbar laminectomy in rabbits

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