Abstract:

【Ａｂｓｔｒａｃｔ】　ＯｂｊｅｃｔｉｖｅTo observe the release of interleukin-6 and tumor necrosis factor-α and the aging of senescent human umbilical vein endothelial cells (HUVECs) induced by angiotensin Ⅱ (AngⅡ).ＭｅｔｈｏｄｓHUVECs were cultured in vitro and the cell viability was observed by CCK-8.HUVECs were intervened by AngⅡand divided into two groups: the control group, AngⅡ group (stimulated with AngⅡ10-6 mol／L for 48 h). Two ageing markers of ageing associated β-galactosidase activity and cell proliferation activity were adopted as main indexes.β-galactosidase activity was evaluated by immunocytochemical method and cell proliferation activity was measured with flowcytometry. The levels of interleukin-6 and tumor necrosis factor-α in the cell culture were determined with ELISA.ＲｅｓｕｌｔｓThe cell viability by AngⅡ-induced cells was (77.15±6.83) ％, the positive cell number of β-gal staining was significantly higher in AngⅡ-induced cells (81.80±0.92) ％ than that in the control cells,The cell cycle was at G0／G1(68.4±3.4)％,suggestting cell senescence. The levels of interleukin-6 and tumor necrosis factor-α were significantly elevated with the AngⅡ (P<0.01).ＣｏｎｃｌｕｓｉｏｎｓAngⅡ induced HUVECs senescence and one of vascular endothelial cell senescence mechanisms may be relevant to the up-regulated levels of interleukin-6 and tumor necrosis factor-α.