Abstract:

Effect of spironolactone on cardiac remodeling in rats with dilated cardiomyopathyBAI Jie. Department of Geriatrics, Affiliated Changhai Hospital of the Second Military Medical University, Shanghai 200433, China; ZHANG Guo-hui, CHEN Rong, SHENG Jia, WANG Jian-fei.Department of Cardiology,the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China
【Ａｂｓｔｒａｃｔ】　ＯｂｊｅｃｔｉｖｅTo study the effect of aldosterone receptor antagonists(spironolactone) on cadiac remodeling in rats with dilated cardiomyopathy.ＭｅｔｈｏｄｓTen normal rats were selected as control group. Meanwhile, forty adriamycin-induced dilated cardiomyopathy (DCM) rats were randomly divided into 4 groups: DCM control group, low-dose group, middle-dose group and high-dose group. The normal control group received physiological saline 10 mg／(kg·d),and three spironolactone groups received spironolactone 10, 20, 40 mg／(kg·d) respectively. Histopathological characteristics were observed, and then collagen volume fractions were calculated. N-terminal popeptide of collagen type Ⅲ (PⅢNP) was measured at the 9th week.ＲｅｓｕｌｔｓThe collagen percents (％)in the middle-dose group (12.13±0.45)% and the high dose group (11.30±1.10)% were more significantly reduced than that in the DCM group (24.33±0.92)% (P＜0.05).The standards of PⅢNP in serum (ng／ml) in the middle-dose group (131.95±9.20ng／ml) and the high-dose group (226.08±22.10 ng／ml) were more significantly reduced than that in the DCM group (234.17±26.60 ng／ml,P＜0.05).ＣｏｎｃｌｕｓｉｏｎｓThe treatment of 20 mg／(kg·d) or 40 mg／(kg·d) spironolactone for 8 weeks can reduce the collagen percent and fibrosis degree of cardiac muscle, and can improve the cardiac compliance. They partly block the renal-angiotention-aldosterone system and balance the disfunction neuroendocrine system when dilated cardiomyopathy happens.
【Key　ｗｏｒｄｓ】spironolactone; dilated cardiomyopathy; myocardia remodeling