Abstract:

ＯｂｊｅｃｔｉｖｅTo investigate the neurotoxicity of bupivacaine (Bup) and the protective effects of αLipoic acid (LA). ＭｅｔｈｏｄｓMouse neuroblastoma N2a cells were employed in the present study. N2a cells were treated with variant concentrations of Bup (0, 300, 600, 900, 1500, 2000 μmol/L) for different durations (12, 24, 36 h). MTT assay and nuclear condensation analysis were used to evaluate the cell viability and apoptosis, respectively. N2a cells were pretreated with LA (0, 100, 300, 500, 1000, 2000 μmol/L) for 12 h, and followed by treatment with Bup (900 μmol/L) for 24 h. Cell viability was measured by MTT assay. Ｒｅｓｕｌｔｓ(1) Bup significantly induced injury in N2a cells, in both dose and timedependent manners. (2) LA markedly attenuated Bupinduced neurotoxicity in N2a cells, with the maximal protective efficiency at the concentration of 500 μmol/L.ＣｏｎｃｌｕｓｉｏｎｓTreatment of Bup could result in substantial injury in N2a cells. Pretreatment with LA could attenuate neurotoxicity induced by Bup.

Key words: bupivacaine, neurotoxicity, α-lipoic acid