Abstract: Objective To analyze the gene polymorphisms of UGT1A1^*28/^*6 in the elderly patients with colorectal cancer by molecular pathological assay. Methods The tissue samples of the elderly patients with colorectal cancer were analyzed by polymerase chain reaction(PCR)-capillary electrophoresis and sequencing. Results The distribution patterns of UGT1A1^*28 and UGT1A1^*6 genotypes in the elderly patients with colorectal cancer were wild type, mutant hybrid type and mutant homozygous type. There were no significant differences in gender, tumor types, tumor T-stage and primary tumor site between the two genetic polymorphisms(P>0.05). Conclusions The distribution patterns of UGT1A1^*28 and UGT1A1^*6 genotypes were not affected by gender, tumor types, tumor T-stage and primary tumor site in the elderly patients with colorectal cancer. UGT1A1^*6 is more susceptible to occur pure mutation than UGT1A1^*28. If a pure mutation occurs at either gene locus, there may be mutational rejection at the other locus.

Key words: colorectal cancer, uridine diphosphate glucuronide transferase 1A1, gene polymorphism, polymerase chain reaction-capillary electrophoresis, Sanger sequencing