Abstract:

ＯｂｊｅｃｔｉｖｅTo detect the gene ffh in streptococcus sanguis, and to analyze the homology of ffh between streptococcus sanguis and streptococcus mutans. ＭｅｔｈｏｄｓStreptococcus sanguis and streptococcus mutans were cultured in anaerobic atmosphere and the genome was obtained by the alkaline method. A pair of primers were designed in the most reserved region of the sequences from GenBank database in order to obtain the unique polymerase chain reaction products.ＲｅｓｕｌｔｓThe unique product was found and obtained in streptococcus sanguis and streptococcus mutans.ＣｏｎｃｌｕｓｉｏｎｓThe gene ffh could be acquired from streptococcus sanguis,and the homology of the acidresistant gene ffh in streptococcus sanguis and streptococcus mutans is high.