Abstract:

【Ａｂｓｔｒａｃｔ】　ＯｂｊｅｃｔｉｖｅTo investigate the mechanisms of inflammatory cytokines network in the development of inflammaging and EF intervention outcome and its mechanism.ＭｅｔｈｏｄｓHealthy male Sprague Dawley rats were divided into 3 groups: 4 m young group with 4 months rats, 24 m aged group with 24 months rats treated with distilled water intragastric administration and 24 m+EF group with 24 months rats treated with EF by intragastric administration for 3 months from 21 months, 10 each group. The gene expression profiles of rat hippocampal tissues from rats in every group were analyzed by using inflammatory cytokines and receptors cDNA microarray,respectively.The differential expressed genes were
screened.At the same time, the levels of inflammagingrelated inflammatory cytokines proteins expressions of TNFα,IL6 and IL10 in rat hippocampal tissues were evaluated by enzyme linked immunosorbent assay. ＲｅｓｕｌｔｓIn rat hippocampal tissues, compared with those in 4 m group, there were 9 differentially upregulated expressed proinflammarory genes and  6 downregulated expressed antiinflammatory genes in 24 m group. Compared with those in 24 m group, there were 8 upregulated expressed antiinflammatory genes and 11 downregulated expressed proinflammatory genes in 24 m+EF group. Compared with those in 4 m group, the levels of TNFα and IL6 in the supernatant from rat hippocampal tissues in 24 m group increased significantly(P＜001). As compared with those in 24 m group, the levels of TNFα and IL6 in the supernatant from rat hippocampal tissues in 24 m+EF group decreased significantly (P＜001). But the level of IL10 in EF+24 m group  was significantly higher than that in 24 m group (P＜001). The ratio of TNFα to IL10 and IL6 to IL10 in 24 m group increased significantly as compared with those in 4 m group(P＜001), and decreased significantly in 24 m+EF group as compared with those in 24 m group(P＜001). ＣｏｎｃｌｕｓｉｏｎｓThere is upregulation of mRNA and protein expressions of proinflammatory cytokines and receptors in the aged rat hippocampus, which may eventually contribute to disequilibrium of the network of pro and antiinflammatory cytokines and receptors and disequilibrium of response of pro and antiinflammatory system and high inflammatory state, which provides new mechanistic insight into inflammaging. EF may interfere in inflammaging via rebuilding the new equilibrium of the network of pro and antiinflammatory cytokines and receptors and equilibrium of response of pro and antiinflammatory system, which might be caused by downregulation of expressions of proinflammatory cytokines and receptors and upregulation of expressions of antiinflammatory cytokines and receptors.